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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
02/12/2005 |
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Data da última atualização: |
21/08/2009 |
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Autoria: |
GASPAR, J. O.; BELINTANI, P.; ALMEIDA, A. M. R.; KITAJIMA, E. W. |
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Título: |
A primer pair allows amplification of part of the 3'-terminal of carlaviruses genome. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Virus Reviews & Research, Rio de Janeiro, v. 10, p. 140, Nov. 2005. Supplement, ref. P-191. |
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Idioma: |
Inglês |
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Notas: |
Edição dos Resumos da XVI National Meeting of Virology, nov. 2005. |
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Conteúdo: |
The genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP gene showed the highest identify (78,3% nt and 93,5% aa) with the isolate H of Cowpea mild mottle virus (AF024628). Our designed primer have the advantage that about 930 nt from the 3' terminus, comprising part of the CP gene, the 11K gene and the terminal untranslated region can be amplified for sequencing, allowing the detection of carlaviruses and identification of unknown viruses as mebers of this virus genus. MenosThe genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP ge... Mostrar Tudo |
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LEADER 02495naa a2200169 a 4500
001 1468611
005 2009-08-21
008 2005 bl --- 0-- u #d
100 1 $aGASPAR, J. O.
245 $aA primer pair allows amplification of part of the 3'-terminal of carlaviruses genome.
260 $c2005
500 $aEdição dos Resumos da XVI National Meeting of Virology, nov. 2005.
520 $aThe genus Carlavirus belongs to the family Flexiviridae 9Adams et al. Arch. Virol. 149:1045, 2004) and has Carnation latent virus (CLV) as the type member. Badge et al. (Eur. J. Plant Pathol. 102:305, 1996) described a primer (named Carla-Uni), which in association with a oligo d (T21) primer, permits the amplification of a ~ 120 nt fragment lovated at the 11 kDa gene and the polyA tract. This PCR amplification has been used as a diagnostic test for carlaviruses. In order to get a longer PCR product, we compared published sequences of the coat protein gene and found that the amino acids sequence GLGVPTE is conserved in almost al the carlavirus sequenced. This allowed us to design a degenerate primer to that region which, when used with a oligo d(T21) primer, produced in a RT-PCR reaction a fragment og ~930 pb from three carlaviruses tested. This new sense primer has the degenerated sequence 5' - GGBYTNGGBGTNCCNACNGA-3', where B= C or G or T, Y = C or T, N = A or C or G or T. The primer pair was used to amplify sequences from Potato virus S, Cole lantent virus (both transmitted by aphids) and Cowpea mild mottle virus (transmitted by whitefly), all producing a fragment of ~930pb. To verify that the DNA fragments generated by PCR were of viral origin, that from Cowpea mild mottle virus was cloned e sequenced. The amplified fragment (936 pb) comprised the coat protein gene, the 11K gene and a short untraslated sequence before de poly(A) tract. The partial sequencing of the CP gene showed the highest identify (78,3% nt and 93,5% aa) with the isolate H of Cowpea mild mottle virus (AF024628). Our designed primer have the advantage that about 930 nt from the 3' terminus, comprising part of the CP gene, the 11K gene and the terminal untranslated region can be amplified for sequencing, allowing the detection of carlaviruses and identification of unknown viruses as mebers of this virus genus.
700 1 $aBELINTANI, P.
700 1 $aALMEIDA, A. M. R.
700 1 $aKITAJIMA, E. W.
773 $tVirus Reviews & Research, Rio de Janeiro$gv. 10, p. 140, Nov. 2005. Supplement, ref. P-191.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 11 | |
| 2. |  | ALMEIDA, A. M. R.; MITUTI, T.; BELINTANI, P.; GASPAR, J. O.; KITAJIMA, E. W. Identificação e caracterização de um carlavirus em Arachys repens Hando. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. p. 310-311. (Embrapa Soja. Documentos, 257). Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 3. | | ALMEIDA, A. M. R.; SAKAI, J.; HANADA, K.; BELINTANI, P.; KITAJIMA, E. W. Caracterização bio-molecular do Tobacco streak virus causador da queima do broto da soja no Brasil. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 27., 2005. Cornélio Procópio. Resumos... Londrina: Embrapa Soja, 2005. p. 308-309. (Embrapa Soja. Documentos, 257). Organizado por Odilon Ferreira Saraiva, Janete Lasso Ortiz, Simone Ery Grosskopf.| Biblioteca(s): Embrapa Soja. |
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| 4. | | GASPAR, J. O.; BELINTANI, P.; ALMEIDA, A. M. R.; KITAJIMA, E. W. A primer pair allows amplification of part of the 3'-terminal of carlaviruses genome. Virus Reviews & Research, Rio de Janeiro, v. 10, p. 140, Nov. 2005. Supplement, ref. P-191. Edição dos Resumos da XVI National Meeting of Virology, nov. 2005.| Biblioteca(s): Embrapa Soja. |
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| 5. | | BELINTANI, P. GASPAR, J. O.; MITUTI, T.; ALMEIDA, A. M.; KITAJIMA, E. W. Sequenciamento parcial e identificação de um carlavirus obtido de Arachys repens. Fitopatologia Brasileira, Brasília, DF, v. 30, p. S 191, ago. 2005. Suplemento. ref. 815. Edição dos Resumos do XXXVIII Congresso Brasileiro de Fitopatologia, Brasília, DF, ago. 2005. Nome correto do quarto autor: ALMEIDA, A. M. R.| Biblioteca(s): Embrapa Soja. |
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| 6. | | BELINTANI, P.; GASPAR, J. O.; ALMEIDA, A. M. R.; KITAJIMA, E. W. Partial sequencing of Cowpea mild mottle virus - CPMMV, isolate of Barreiras - Bahia. Virus Reviews & Research, Rio de Janeiro, v. 10, p. 31, Nov. 2005. Supplement. Edição dos Resumos da XVI National Meeting of Virology, nov. 2005.| Biblioteca(s): Embrapa Soja. |
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| 7. | | MITUTI, T.; CORTEZI, D.; NUNES, C. L.; KITAJIMA, E. W.; BELINTANI, P.; GASPARJ. O.; ALMEIDA, A. M. R. Identificação de um isolado de carlavirus em Arachis pintoi Krap & Greg. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2005, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2005. p. 140-144. (Embrapa Soja. Documentos, 268).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 8. | | MITUTI, T.; CORTEZI, D. G.; NUNES, C. L.; KITAJIMA, E. W.; BELINTANI, P.; GASPAR, J. O.; ALMEIDA, A. M. Identificação de um isolado de carlavirus em Arachis repens Handro. Fitopatologia Brasileira, Brasília, DF, v. 30, p. S 187, ago. 2005. Suplemento. ref. 788. Edição dos Resumos do XXXVIII Congresso Brasileiro de Fitopatologia, Brasília, DF, ago. 2005. Nome correto do sétimo autor: ALMEIDA, A. M. R.| Biblioteca(s): Embrapa Soja. |
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| 9. | | ALMEIDA, A. M. R.; SAKAI, J.; HANADA, K.; OLIVEIRA, T. G.; BELINTANI, P.; KITAJIMA, E. W.; NORA, P. S.; NOVAES, T. G. Biological and molecular characterization of an isolate of tobacco streak virus isolated from bud blight soybeans in Brazil. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 229-230. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.| Biblioteca(s): Embrapa Soja. |
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| 10. | | ALMEIDA, A. M. R.; SAKAI, J.; OLIVEIRA, T. G. O.; BELINTANI, P.; KITAJIMA, E. W.; SOUTO, E. R.; NOVAES, T. G. de; NORA, P. S. Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil. Fitopatologia Brasileira, Brasília, v. 30, n. 4, p. 366-373, July/Aug. 2005.| Biblioteca(s): Embrapa Soja. |
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| 11. | | ALMEIDA, A. M. R.; PIUGA, F. F.; KITAJIMA, E. W.; GASPAR, J. O.; VALENTIN, N.; BENATO, L. C.; MARIN, S. R. R.; BINNECK, E.; OLIVEIRA, T. G. de; BELINTANI, P.; GUERZONI, R. A.; NUNES JUNIOR, J.; HOFFMANN, L.; NORA, P. S.; NEPOMUCENO, A. L.; MEYER, M. C.; ALMEIDA, L. A. Necrose da haste da soja. Londrina: Embrapa Soja, 2003. 44 p. (Embrapa Soja. Documentos, 221).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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